CelLytic ^TM MEM 蛋白提取試劑盒

NA.56

膜蛋白占據了生物體基因組大約20-30%的成分，充當著胞內的看門人、調節器和感應器的角色。它們具有多樣的細胞功能，例如將細胞與外部的毒素隔離開來，作為胞內信號級聯的起始點，以及維持臨界離子濃度。
CelLytic^™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.

CelLytic^™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.

CelLytic is a trademark of sigma-aldrich Co. LLC

組份不可單獨銷售
Lysis and Separation Buffer 50 mL
Wash Buffer for CelLytic MEM 50 mL
Sodium Chloride, 4M Solution 1.5 mL

200

wet ice

−20℃

提供足夠進行80次檢測的試劑。

10 - Combustible liquids

WGK 2

188.6 °F - closed cup

87 ℃ - closed cup