您的位置：首頁 > 產品中心 > SYBR^® Green Extract-N-Amp^TM組織PCR試劑盒

SYBR^® Green Extract-N-Amp^TM組織PCR試劑盒

SYBR^® Green Extract-N-Amp^TM Tissue PCR Kit

產品編號：

3246201

產品類別：

試劑盒

純度規格：

sufficient for 100 extractions, sufficient for 100 amplifications

包裝規格：

1 KIT

市場價：

4500元

優惠價：

立即咨詢

SYBR^® Green Extract-N-Amp^TM組織PCR試劑盒

SYBR^® Green Extract-N-Amp^TM Tissue PCR Kit

產品性質

usage【用途】	sufficient for 100 amplifications sufficient for 100 extractions sufficient for 100 reactions
Quality Level【質量水平】	200
feature【特點】	dNTPs included hotstart
technique(s)	PCR: suitable
web metadata keyword.default	XNATRG
storage temp.【儲存溫度】	−20℃

基本信息

General description【一般描述】	The SYBR^® Green Extract-N-Amp^™ Tissue PCR kit is a direct PCR (polymerase chain reaction) kit that contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Application【應用】	Extract-N-Amp Video Protocol SYBR^® Green Extract-N-Amp^™ tissue PCR kit has been used for DNA extraction from embryo head of male Pmp22 transgenic Sprague–Dawley rats and in genotyping. This direct PCR (polymerase chain reaction) kit is also suitable for gene copy number experiments and amplifying and quantifying DNA from multiple tissue sample types.
Features and Benefits【特點和優勢】	Novel – all liquid, single-step extraction of genomic DNA for quantitative PCR (qPCR) Fast – tissue to qPCR in 15 minutes Convenient – no long enzymatic digestions and no column purifications Simple – rapid, easy-to-follow protocol Sensitive – specially formulated Hot Start^® SYBR^® Green PCR ReadyMix^™ for highly specific PCR amplification and quantitation of genomic DNA Safe – no organic extraction with hazardous chemicals
Components【組分】	試劑盒含有Extraction Solution、Neutralization Solution、Tissue Preparation Solution和Extract-N-Amp SYBR Green PCR ReadyMix^™。Extract-N-Amp SYBR Green PCR ReadyMix為2X反應混合物，其中含有 SYBR Green、緩沖液、鹽、dNTP、Taq聚合酶和 JumpStart^™ Taq 抗體。此試劑盒專為結合提取試劑使用進行了優化，其中含有適用于熱啟動PCR、可增強特異性的 JumpStart Taq抗體，以及可用作實時定量PCR的非特異性報告標志物的SYBR Green I。
Principle【原理】	DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances and the extract is ready for real-time PCR in any plate-based real-time thermal cycling system. An aliquot of the neutralized extract is then combined with the Extract-N-Amp^™ SYBR^® Green PCR ReadyMix^™ and user-provided PCR primers.
Other Notes【其他說明】	更多信息，請見www.sigma-aldrich.com/extract-n-amp。
Legal Information【法律信息】	本產品的使用受到如下一項或多項美國專利及其對應的境外專利聲明保護：5,994,056和6,171,785。購買本產品，即相當于依照境外的專利聲明獲得了一份受限制、不可轉讓的，僅此等數量的產品用于購買者內部研究用途的的免責許可。其他專利聲明下的任何權利(如美國專利號6,814,934號下的設備和系統專利聲明)、進行任何專利方法申請的權利、進行任何形式的商業服務的權利，包括但不限于出于收費或其他商業考慮而報告購買者的研究活動結果的權利。本產品僅適合于研究用途。如需用于Roche專利許可的診斷用途，需另外征得Roche許可。有關購買許可的更多信息，可聯系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA獲取。依照美國專利號5,338,671和5,587,287和其他地區對應的專利，許可抗體用于體外研究用途。 Extract-N-Amp is a trademark of sigma-aldrich Co. LLC HOTSTART is a registered trademark of Molecular BioProducts, Inc. JumpStart is a trademark of sigma-aldrich Co. LLC ReadyMix is a trademark of sigma-aldrich Co. LLC SYBR is a registered trademark of Life Technologies

安全信息

Pictograms【象形圖】	GHS07,GHS08,GHS09
Signal word【警示用語：】	Danger
Hazard Statements	H315 - H317 - H319 - H334 - H335 - H411
Precautionary Statements	P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
Hazard Classifications【危險分類】	Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs【靶器官】	Respiratory system
Storage Class Code【儲存分類代碼】	10 - Combustible liquids
WGK	WGK 3

SYBR® Green Extract-N-AmpTM組織PCR試劑盒

SYBR^® Green Extract-N-Amp^TM組織PCR試劑盒